Application

Detect Spred-1 using this mouse monoclonal antibody, Anti-Spred-1 Antibody, clone M23-P2G3 validated for use in western blotting & ICC.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunocytochemistry Analysis: A representative lot detected Spred-1 in HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) is a tyrosine kinase substrate which has a cysteine-rich domain which resembles the SPR domain at the carboxy terminus. SPRED1 associated with Ras blocks phosphorylation of Raf and disrupts MAP kinase signaling during the differentiation of neuronal cells and myocytes. A mutation in SPRED1 has been linked to Lugius syndrome, an autosomal dominant RAS-MAPK pathway syndrome.

Immunogen

Linear peptide corresponding to human Spred-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Spred-1 in 10 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 60 kDa observed